NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE143462 Query DataSets for GSE143462
Status Public on Jan 11, 2020
Title Omipalisib Inhibits Esophageal Cancer Growth through PI3K/AKT/mTOR Signalling Pathway Disruption
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Esophageal squamous cell carcinoma (OSCC) is a lethal digestive tract malignancy with limited effective interventions. This study aims to explore a new potential inhibitor on OSCC and investigate the underlying mechanisms. Based on a drug screen of an FDA-proven library consisting of 1404 compounds, we demonstrated that Omipalisib, a dual PI3K/mTOR inhibitor, markedly inhibited cell proliferation and colony formation in a panel of OSCC cell lines. Mechanistically, Omipalisib induced G0/G1 phase cell cycle arrest and apoptosis. Moreover, RNA-seq, KEGG and GSEA confirmed that the PI3K/mTOR signalling pathway is the primary target of Omipalisib in OSCC cells. Omipalisib treatment suppressed the PI3K/mTOR signalling pathway. Furthermore, Omipalisib exerted a significant antineoplastic effect on OSCC tumour xenografts in BALB/c nude mice without obvious side effects. The present study supports the rationale for using Omipalisib as a therapeutic approach for OSCC cases and suggests the potential clinical evaluation of this strategy.
 
Overall design In this study, we investigated the therapeutic effects and underlying mechanisms of Omipalisib in OSCC cells based on a screen of compound library.
 
Contributor(s) Zhu D, Dong J, Xu Y, Zhang X, Fu S, Liu W
Citation(s) 32804918
Submission date Jan 10, 2020
Last update date Sep 08, 2020
Contact name Dongshan Zhu
E-mail(s) [email protected]
Phone 13643720644
Organization name Jilin University First Hospital
Department thracic department
Street address 71 Xinmin Street
City Changchun Jilin
ZIP/Postal code 130021
Country China
 
Platforms (1)
GPL23227 BGISEQ-500 (Homo sapiens)
Samples (6)
GSM4259875 Con-150-1-1
GSM4259876 Con-150-2-1
GSM4259877 Con-150-3-1
Relations
BioProject PRJNA600533
SRA SRP241240

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143462_gene_expression.txt.gz 1.2 Mb (ftp)(http) TXT
GSE143462_rna_expression.txt.gz 6.8 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap