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| Status |
Public on Dec 22, 2009 |
| Title |
Human epithelial cell response to bacterial signal indole |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Indole is a bacterial signal secreted by pathogenic and commensal Escherichia coli in the stationary phase at high concentrations (~600 µM). Prior work from our lab has shown that indole decreases E. coli O157:H7 (EHEC) chemotaxis, motility, attachment to epithelial cells and biofilm formation. However, its effect on epithelial cells is not known. We hypothesized that indole induces gene expression changes in epithelial cells that lead to decreased pathogen colonization and infection. Changes in gene expression with the human enterocyte cell line HCT-8 exposed to indole suggested down-regulation of Toll-like receptor signaling and coordinated changes in Jak-STAT and p38 MAPK pathways. Corresponding changes in the expression of cytokines, chemokines, and their receptors also suggested that indole functions as a modulator of inflammation in intestinal epithelial cells. In addition, the expression of genes involved in tight junction organization (claudins, and tight junction proteins) and mucin production were also up-regulated by indole.
Keywords: Inter-kingdom signaling interactions between human cells and bacterial signal indole
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| Overall design |
Human intestinal epithelial cells were exposed to 1 mM indole for 4 h or 24 h. RNA was isolated from the control cells and cells exposed to indole for 4 h and 24 h. The experiments were performed in triplicate. A total of 9 microarrays were performed, three for each condition. The gene expression data was analyzed using significance analysis of microarrays (SAM) where a false discovery rate cut-off of 1% was used. The genes were then classified and importnat regulated pathways were reported.
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| |
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| Contributor(s) |
Bansal T, Wood TK, Jayaraman A |
| Citation(s) |
19966295 |
| |
| Submission date |
Jan 12, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Tarun Bansal |
| E-mail(s) |
[email protected]
|
| Phone |
979-661-4318
|
| Organization name |
Texas A & M University
|
| Department |
Chemical Engineering
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| Lab |
Prof Thomas Wood Lab
|
| Street address |
3122 TAMU
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8060 |
Texas A&M CodeLink Human Whole Genome Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA111427 |
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