|
| Status |
Public on Mar 06, 2020 |
| Title |
Identifying candidate genes whose expression is regulated by the HutC transcriptional repressor in Pseudomonas fluorescens SBW25 |
| Organism |
Pseudomonas fluorescens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
HutC in Pseudomonas is a representative member of the HutC/GntR family of transcriptional regulators, which possess a N-terminal winged helix-turn-helix (wHTH) DNA-binding domain and a C-terminal substrate binding domain. HutC is generally known to represses expression of histidine utilization (hut) genes through binding to the PhutU promoter with urocanate (the first intermediate of the histidine degradation pathway) as the direct inducer. Here we first describe the detailed molecular interactions between HutC and its PhutU target site in a plant growth promoting bacterium P. fluorescens SBW25, and further show that HutC possesses specific DNA-binding activities with many targets in SBW25 genome. Subsequent RNA-seq analysis and phenotypic assays revealed an unexpected global regulatory role of HutC for successful bacterial colonization in planta.
|
| |
|
| Overall design |
Gene expression was compared between wild-type P. fluorescens SBW25 and its derived hutC deletion mutant.
|
| |
|
| Contributor(s) |
Zhang X |
| Citation(s) |
32291279 |
| |
| Submission date |
Mar 05, 2020 |
| Last update date |
Jul 04, 2020 |
| Contact name |
Xue-Xian Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Massey University
|
| Department |
School of Natural and Computational Sciences
|
| Street address |
14.02 The Station Cre., Albany
|
| City |
Auckland |
| ZIP/Postal code |
0745 |
| Country |
New Zealand |
| |
|
| Platforms (1) |
| GPL28231 |
Illumina HiSeq 4000 (Pseudomonas fluorescens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA610657 |
| SRA |
SRP251781 |
