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| Status |
Public on Mar 12, 2020 |
| Title |
Common regulatory targets of NFIA, NFIX and NFIB during postnatal cerebellar development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptional regulation plays a central role in controlling neural stem and progenitor cell proliferation and differentiation during neurogenesis. For instance, transcription factors from the nuclear factor I (NFI) family have been shown to co-ordinate neural stem and progenitor cell differentiation within multiple regions of the embryonic nervous system, including the neocortex, hippocampus, spinal cord and cerebellum. Knockout of individual Nfi genes culminates in similar phenotypes, suggestive of common target genes for these transcription factors. However, whether or not the NFI family regulates common suites of genes remains poorly defined. Here, we use granule neuron precursors (GNPs) of the postnatal murine cerebellum as a model system to analyse regulatory targets of three members of the NFI family: NFIA, NFIB and NFIX. By integrating transcriptomic profiling (RNA-seq) of Nfia- and Nfix-deficient GNPs with epigenomic profiling (ChIP-seq against NFIA, NFIB and NFIX, and DNase I hypersensitivity assays), we reveal that these transcription factors share a large set of potential transcriptional targets, suggestive of complementary roles for these NFI family members in promoting neural development.
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| Overall design |
Genome-wide ChIP-seq analysis of the binding of NfiX (Sigma Aldrich, SAB1401263), NfiA (Abcam, ab41851) and NfiB (Sigma HPA003956) on the genome in granule neuron progenitor cells isolated from the cerebellum of postnatal day 7 CD1 mice.
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| Contributor(s) |
Brown A, Scott M |
| Citation(s) |
31838646 |
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| Submission date |
Mar 11, 2020 |
| Last update date |
Mar 18, 2020 |
| Contact name |
Mikael Boden |
| E-mail(s) |
[email protected]
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| Phone |
+61733651307
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| Organization name |
The University of Queensland
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| Department |
School of Chemistry and Molecular Biosciences
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| Lab |
Boden
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| Street address |
Cooper Rd
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| City |
St Lucia |
| State/province |
Queensland |
| ZIP/Postal code |
4072 |
| Country |
Australia |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA611985 |
| SRA |
SRP252404 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE146793_RAW.tar |
3.5 Mb |
(http)(custom) |
TAR (of NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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