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| Status |
Public on Sep 01, 2023 |
| Title |
Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [MITRG 10X data] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used single-cell RNA-sequencing to investigate the role of myeloid cells in a humanized mouse model of breast cancer brain metastasis (BCBM). We generated brain metastases with a cardiac-injection of the human triple-negative breast cancer cell line MDA-MB-231BR2 (231BR) into MITRG mice and allowed tumors to grow in the brain for 25 days. We then took all human cells (includes myeloid cells and 231BR) from these mice verses control MITRG brains (only myeloid cells) to find transcriptional changes associated with BCBM.
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| Overall design |
Single-cell RNA-Seq of human iPSC-derived myeloid cells and MDA-MB-231BR2 from the brains of MITRG mice with and without breast cancer brain metastases.
Please note that, as both BAM and FASTQ are necessary to interpret the data, duplicated sample records are created to link two different raw data type (i.e. BAM containing cDNA reads and fastq containing a set of DNA barcodes from the MULTI-seq element)
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| |
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| Contributor(s) |
Lawson DA, Blake K |
| Citation(s) |
37957324 |
| |
| Submission date |
Apr 01, 2020 |
| Last update date |
Dec 04, 2023 |
| Contact name |
Devon Lawson |
| Organization name |
University of California, Irvine
|
| Street address |
839 Health Science Road, Sprague Hall 140
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92612 |
| Country |
USA |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL25526 |
Illumina NovaSeq 6000 (Homo sapiens; Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE147949 |
Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis |
|
| Relations |
| BioProject |
PRJNA622587 |
| SRA |
SRP254951 |
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