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| Status |
Public on Apr 20, 2020 |
| Title |
ClpV3 affects pathogenicity in Pseudomonas aeruginosa |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
ClpV3 is a cytoplasmic AAA+ ATPase protein and is an essential component of H3-T6SS in Pseudomonas aeruginosa. Here, we report that an H3-T6SS deletion mutant PAO1(ΔclpV3) significantly affected the virulence-related phenotypes including pyocyanin production, biofilm formation, proteolytic activity and motilities. Most interestingly, the expression of T3SS genes was markedly affected, indicating a strong link between H3-T6SS and T3SS. RNA-Sequencing was performed to globally identify the genes differentially expressed when H3-T6SS was inactivated and the results obtained could be well correlated to the observed phenotypes.
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| |
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| Overall design |
Gene expression was compared between wild-type P. aeruginosa PAO1 and clpV3 deletion mutant.
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| |
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| Contributor(s) |
Duan K |
| Citation(s) |
32547522 |
| |
| Submission date |
Apr 06, 2020 |
| Last update date |
Jul 21, 2020 |
| Contact name |
Kangmin Duan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Manitoba
|
| Street address |
66 Chancellors Cir
|
| City |
Winnipeg |
| State/province |
MB |
| ZIP/Postal code |
R3T 2N2 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL18782 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA626445 |
| SRA |
SRP257374 |
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