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| Status |
Public on Jun 10, 2009 |
| Title |
Disease-specific expression of host genes during downy mildew infection of Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Here we report on the identification of Arabidopsis genes that are induced during compatible but not during incompatible interactions with the downy mildew pathogen Hyaloperonospora arabidopsidis. This set of so-called compatible specific (CS) genes contrasts the large group of defence-associated genes that is differentially expressed during both compatible and incompatible interactions. From the 17 identified CS-genes, 6 belong to the ERF family of transcription factor genes, suggesting that these ERFs have a role during compatibility. The majority of CS-genes are differentially regulated in response to various forms of abiotic stress. In silico analysis of the CS-genes revealed an over-representation of DREB1A/CBF3 binding sites and EveningElement motifs in their promoter regions. The CS-ERFs are closely related to the CBF transcription factors and could potentially bind the DREB1A/CBF3 promoter elements in the CS-genes. Transcript levels of CS-genes peak at 2-3 days post inoculation, when pathogen growth is highest, and decline at later stages of infection. The induction of several CS-genes was found to be isolate-dependant. This suggests that the identified CS-genes could be the direct or indirect targets of downy mildew effector proteins that promote disease susceptibility.
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| Overall design |
Compatible interaction - Arabidopsis Ler inoculated with Cala2 compared to Ler with mock inoculation with 3 biological replicates. Each biological replicate is analysed by 4 technical dyeswapped replicates.
Incompatible interaction - Arabidopsis Ler inoculated with Waco9 compared to Ler with mock inoculation with 3 biological replicates. Each biological replicate is analysed by 4 technical dyeswapped replicates.
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| Contributor(s) |
Huibers R, de Jong M, Dekter R, Van den Ackerveken G |
| Citation(s) |
19656045 |
| |
| Submission date |
Feb 23, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Mark de Jong |
| Organization name |
University of Amsterdam
|
| Street address |
Kruislaan 318
|
| City |
Amsterdam |
| ZIP/Postal code |
1098 SM |
| Country |
Netherlands |
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| Platforms (1) |
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| Samples (6)
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| GSM373298 |
Differentially expressed genes in the Arabidopsis Ler-Cala2 interaction - Biological replicate 1 |
| GSM373299 |
Differentially expressed genes in the Arabidopsis Ler-Cala2 interaction - Biological replicate 2 |
| GSM373320 |
Differentially expressed genes in the Arabidopsis Ler-Cala2 interaction - Biological replicate 3 |
| GSM373321 |
Differentially expressed genes in the Arabidopsis Ler-Waco9 interaction - Biological replicate 1 |
| GSM373322 |
Differentially expressed genes in the Arabidopsis Ler-Waco9 interaction - Biological replicate 2 |
| GSM373323 |
Differentially expressed genes in the Arabidopsis Ler-Waco9 interaction - Biological replicate 3 |
|
| Relations |
| BioProject |
PRJNA111993 |
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