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| Status |
Public on Apr 20, 2021 |
| Title |
Effect of flg22 peptide over transcriptome and nucleosome landscape of Arabidopsis on Col-0 and chromatin remodelling ATPase EDA16 mutants |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Activation of plant immunity by bacterial MAMPs such as flg22 affects nucleosome positioning over thousands of loci and is correlated with the transcription of immune-related genes. Data for chromatin remodelling ATPase EDA16 truncated and over-expressor mutants as well as Col-0 (control) are provided.
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| Overall design |
2-week-old seedlings were treated with 100 nM flg22 or mock-treated for 2 hours. Material was frozen and ground in liquid nitrogen splitting sample into 2 batches; one for RNA-seq and one for MNase-seq. Col-0, eda16-OE (SAIL_40_F09) and eda16-∆Hc (SAIL_735_G06) seedlings were used. This experiment was carried out in duplicate with 24 samples in total (12 samples for RNA-seq and 12 samples MNase-seq). Samples single-end sequenced with NextSeq 500 Illumina sequencer
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| Contributor(s) |
Pardal AJ, Piquerez SJ, Concia L, Benhamed M, Ntoukakis V |
| Citation(s) |
34015058 |
| |
| Submission date |
Apr 30, 2020 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Lorenzo Concia |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
769929568
|
| Organization name |
École Normale Supérieure
|
| Department |
Institut de Biologie
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| Lab |
Plant and Algal Genomics Lab
|
| Street address |
46 Rue d'Ulm
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| City |
Paris |
| ZIP/Postal code |
75020 |
| Country |
France |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA629545 |
| SRA |
SRP259350 |
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