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| Status |
Public on Feb 25, 2021 |
| Title |
The gut microbiome regulates memory function. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The mammalian gastrointestinal tract contains a diverse ecosystem of microbial species collectively making up the gut microbiome. Emerging evidence highlights a critical relationship between gut microbiota and neurocognitive development. Consumption of unhealthy yet palatable dietary factors associated with obesity and metabolic dysfunction (e.g., saturated fat, added sugar) produces microbiota dysbiosis and negatively impacts neurocognitive function, particularly when consumed during early life developmental periods. Here we explore whether excessive early life consumption of added sugars negatively impacts neurocognitive development via the gut microbiome. Using a rodent model of habitual sugar-sweetened beverage (SSB) consumption during the adolescent stage of development, we first show that excessive early life sugar intake impairs hippocampal-dependent memory function when tested during adulthood while preserving other neurocognitive domains. Gut microbiome genomic sequencing analyses reveal that early life SSB consumption alters the abundance of various bacterial populations, including elevations in operational taxonomic units within the genus Parabacteroides (P. distasonis and P. johnsonii) whose abundance negatively correlated with memory task performance. Additional results reveal that in vivo Parabacteroides enrichment of cultured P. distasonis and P. johnsonii bacterial species in adolescent rats severely impairs memory function during adulthood. Hippocampus transcriptome analyses identify gene expression alterations in neurotransmitter synaptic signaling, intracellular kinase signaling, metabolic function, neurodegenerative disease, and dopaminergic synaptic signaling-associated pathways as potential mechanisms linking microbiome outcomes with memory impairment. Collectively these results identify microbiota dysbiosis as a mechanism through which early life unhealthy dietary patterns negatively impact neurocognitive outcomes.
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| Overall design |
Two experimental desiign were involved. First one is compare hiippocampus responses beetween rats given sugar water (65% fructose and 35% sucrose, SUG group). Second one is to compare hippocampus response in the following treatments. Rats treated with saline for 21 days (Sal_Sal), Rats treated with antibiotics cocktail (Vancomycin (50 mg/kg), Neomycin (100 mg/kg), and Metronidazole (100 mg/kg) ) along with 1 mg/mL of ampicillin in their drinking water) for 7 days followed by saline till PN 83 (Abx_Sal), Rats treated by antibiotcs cocktail for 7 days and 1:1 ratio of Parabacteroides distasonis and Parabacteroides johnsonii gavage till PN 83(Abx_Pb)
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/33790226/
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| |
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| Contributor(s) |
Kanoski SE, Noble EE |
| Citation(s) |
33790226 |
| |
| Submission date |
May 07, 2020 |
| Last update date |
Jun 30, 2023 |
| Contact name |
Yen-Wei Chen |
| E-mail(s) |
[email protected]
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| Organization name |
University of California, Los Angeles
|
| Department |
IDP Molecular Toxicology
|
| Street address |
650 Charles E. Young Dr. South 16-035 Center for Health Sciences
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL25029 |
NextSeq 550 (Rattus norvegicus) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA631097 |
| SRA |
SRP260872 |
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