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| Status |
Public on May 17, 2021 |
| Title |
Total RNA sequencing of the liver tissues of Oreochromis niloticus (Nile Tilapia) and of Pundamilia nyerei |
| Organisms |
Oreochromis niloticus; Pundamilia nyererei |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cichlids fishes exhibit extensive phenotypic diversification and speciation. In this study we integrate transcriptomic and proteomic signatures from two cichlids species, identify novel open reading frames (nORFs) and perform evolutionary analysis on these nORF regions. We embark comparative transrcriptomics and proteogenomic analysis of two metabolically active tissues, the testes and liver, of two cichlid species Oreochromis niloticus (Nile tilapia, ON) and Pundamilia nyererei (Makobe Island, PN). Our results suggest that the time scale of speciation of the two species can be better explained by the evolutionary divergence of these nORF genomic regions.
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| Overall design |
Total RNA was extracted from liver tissue of two cichlid fishes: Oreochromis niloticus and Pundamilia nyererei; three samples each.
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| Contributor(s) |
Puntambekar SS, Newhouse R, Navas JS, Chauhan R, Vernaz G, Willis T, Wayland MT, Urmania Y, Miska EA, Prabakaran S |
| Citation(s) |
33299045 |
| |
| Submission date |
May 18, 2020 |
| Last update date |
Aug 23, 2021 |
| Contact name |
Sudhakaran Prabakaran |
| Organization name |
University of Cambridge
|
| Department |
Department of Genetics
|
| Street address |
Downing Site, CB2 3EH, UK
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| City |
Cambridge |
| ZIP/Postal code |
CB2 3EH |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL23067 |
Illumina HiSeq 2500 (Oreochromis niloticus) |
| GPL28548 |
Illumina HiSeq 2500 (Pundamilia nyererei) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA633492 |
| SRA |
SRP262128 |
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