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| Status |
Public on Jul 31, 2020 |
| Title |
The ISW1a ATP-dependent chromatin remodeler acts specifically on dinucleosomes to regulate early transcription [MNase-Seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The large family of ATP-dependent chromatin remodelers that regulate chromatin organization and composition are thought to act only on individual mononucleosomes. We discovered however that the yeast ISW1a remodeler has a much higher affinity for dinucleosomes and only efficiently converts ATP hydrolysis into nucleosome movement when bound to dinucleosomes, not mononucleosomes. The Ioc3 accessory subunit and its HLB DNA binding domain confer dinucleosome specificity onto the Isw1 catalytic subunit. Perturbation of ISW1a dinucleosome specificity by mutating Ioc3 interferes with its localization immediately downstream of the early transcription complex. ISW1a and NuA4, a histone acetyl transferase, and SWR1, a histone exchanger, are required together to slow early passage of RNA polymerase II. These findings provide new insights into the unusual specificity of a chromatin remodeler regulating the transition from transcription initiation to elongation and its functional interplay with other chromatin modifiers.
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| Overall design |
Nine samples (WT ISW1a, △Ioc3 and Ioc3△cHLB) are compared in triplicates (MNase-seq).
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| |
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| Contributor(s) |
Hailu SG, Bartholomew B |
| Citation(s) |
33219211 |
| |
| Submission date |
May 19, 2020 |
| Last update date |
Dec 01, 2020 |
| Contact name |
Blaine Bartholomew |
| E-mail(s) |
[email protected]
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Epigenetics and Molecular Carcinogenesis
|
| Lab |
Bartholomew
|
| Street address |
1808 Park Road 1C
|
| City |
Smithville |
| ZIP/Postal code |
78957 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE150829 |
The ISW1a ATP-dependent chromatin remodeler acts specifically on dinucleosomes to regulate early transcription |
|
| Relations |
| BioProject |
PRJNA633799 |
| SRA |
SRP262297 |
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