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| Status |
Public on May 21, 2020 |
| Title |
Transcriptomic Comparison of Common Nontyphoidal Salmonella Serovars Provides Greater Resolution for Understanding Differences in Virulence |
| Organisms |
Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella enterica subsp. enterica serovar Cerro; Salmonella enterica subsp. enterica serovar Javiana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA extracted from representative isolates of each serovar grown to late exponential phase in Luria-Bertani broth showed that transcript abundances of core genes were significantly higher (p < 0.05) than those of accessory genes for all three serovars. Inter-serovar comparisons demonstrated higher transcript abundances for genes related to vitamin B12 biosynthesis, and ethanolamine and 1,2-propanediol utilization in S. Cerro compared to Javiana and Typhimurium, while genes in Salmonella Pathogenicity Island (SPI) 1 had significantly higher transcript abundances in S. Javiana and S. Typhimurium.
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| Overall design |
mRNA profiles from three Salmonella serovars (Cerro, Javiana, Typhimurium) were generated by deep sequencing with an Illumina NextSeq 500 in triplicate with 75-nt stranded single-end reads.
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| Contributor(s) |
Cohn A, Orsi RH, Carroll L, Cheng R, Wiedmann M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 20, 2020 |
| Last update date |
May 24, 2020 |
| Contact name |
Alexa Robyn Cohn |
| E-mail(s) |
[email protected]
|
| Organization name |
Cornell University
|
| Department |
Food Science
|
| Lab |
Wiedmann
|
| Street address |
411 Tower Rd
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (3) |
| GPL21220 |
Illumina NextSeq 500 (Salmonella enterica subsp. enterica serovar Typhimurium) |
| GPL28563 |
Illumina NextSeq 500 (Salmonella enterica subsp. enterica serovar Cerro) |
| GPL28564 |
Illumina NextSeq 500 (Salmonella enterica subsp. enterica serovar Javiana) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA634123 |
| SRA |
SRP262500 |
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