|
| Status |
Public on Jun 06, 2020 |
| Title |
Replacing the Calvin cycle with the reductive glycine pathway in Cupriavidus necator |
| Organism |
Cupriavidus necator |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
These data belong to a metabolic engineering project that introduces the reductive glycine pathway for formate assimilation in Cupriavidus necator. As part of this project we performed short-term evolution of the bacterium Cupriavidus necator H16 to grow on glycine as sole carbon and energy source. Some mutations in a putiative glycine transporting systems facilitated growth, and we performed transcriptomics on the evolved strain growing on glycine. Analysis of these transcriptomic data lead us to the discovery of a glycine oxidase (DadA6), which we experimentally demonstrated to play a key role in the glycine assimilation pathay in C. necator.
|
| |
|
| Overall design |
Transcriptomes are performed for the evolved strain growing on glyine (in triplicate).
|
| |
|
| Contributor(s) |
Claassens NJ, Bar-Even A |
| Citation(s) |
32805426 |
| |
| Submission date |
Jun 05, 2020 |
| Last update date |
Sep 07, 2020 |
| Contact name |
Nico J Claassens |
| E-mail(s) |
[email protected]
|
| Phone |
0317483740
|
| Organization name |
Wageningen University
|
| Department |
Laboratory of Microbiology
|
| Street address |
Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
6708 WE |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL27909 |
Illumina HiSeq 3000 (Cupriavidus necator) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA637590 |
| SRA |
SRP266134 |
