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| Status |
Public on Dec 23, 2020 |
| Title |
H-NS-like proteins in Pseudomonas aeruginosa coordinately silence intragenic transcription [ChIP-seq] |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The H-NS-like proteins MvaT and MvaU act coordinately as global repressors in Pseudomonas aeruginosa by binding to AT-rich regions of the chromosome. Although cells can tolerate the loss of either protein, identifying their combined regulatory effects has been challenging because the loss of both proteins is lethal due to induction of prophage Pf4 and subsequent superinfection of the cell. In other bacteria, H-NS promotes cellular fitness by inhibiting intragenic transcription from AT-rich target regions, preventing them from sequestering RNA polymerase; however, it is not known if whether MvaT and MvaU function similarly. Here we utilize a parental strain that cannot be infected by Pf4 phage to define the collective MvaT and MvaU regulon and demonstrate that the combined loss of both MvaT and MvaU leads to increased intragenic transcription from loci directly controlled by these proteins. We further show that the loss of MvaT and MvaU leads to a striking redistribution of RNA polymerase containing σ70 to genomic regions vacated by these proteins. Our findings suggest that the ability of H-NS-like proteins to repress intragenic transcription is a universal function of these proteins and point to a second mechanism by which MvaT and MvaU may contribute to the growth of P. aeruginosa.
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| Overall design |
Genome-wide locations of RpoD in a parental strain lacking pilY1 and a strain with additional deletions of mvaU and mvaT. Also, genome-wide locations of MvaT and MvaU in wild-type cells.
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| |
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| Contributor(s) |
Lippa AM, Gebhardt MJ, Dove SL |
| Citation(s) |
33245158 |
| |
| Submission date |
Jul 23, 2020 |
| Last update date |
Mar 24, 2021 |
| Contact name |
Andrew M Lippa |
| Organization name |
Boston Children's Hospital
|
| Department |
Pediatrics, Division of Infectious Diseases
|
| Lab |
Dove Lab
|
| Street address |
300 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18782 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1) |
|
| Samples (21)
|
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| This SubSeries is part of SuperSeries: |
| GSE155025 |
H-NS-like proteins in Pseudomonas aeruginosa coordinately silence intragenic transcription |
|
| Relations |
| BioProject |
PRJNA648196 |
| SRA |
SRP273351 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155024_MvaT-V_A_background_normalized.profile.wig.gz |
20.5 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaT-V_A_normalized.profile.wig.gz |
22.4 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaT-V_B_background_normalized.profile.wig.gz |
20.4 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaT-V_B_normalized.profile.wig.gz |
21.8 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaT-V_C_background_normalized.profile.wig.gz |
20.4 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaT-V_C_normalized.profile.wig.gz |
22.2 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaT_AllPeaks.bed.gz |
3.6 Kb |
(ftp)(http) |
BED |
| GSE155024_MvaU-V_A_background_normalized.profile.wig.gz |
20.5 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaU-V_A_normalized.profile.wig.gz |
20.7 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaU-V_B_background_normalized.profile.wig.gz |
20.3 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaU-V_B_normalized.profile.wig.gz |
21.5 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaU-V_C_background_normalized.profile.wig.gz |
20.5 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaU-V_C_normalized.profile.wig.gz |
20.2 Mb |
(ftp)(http) |
WIG |
| GSE155024_MvaU_AllPeaks.bed.gz |
3.6 Kb |
(ftp)(http) |
BED |
| GSE155024_RpoD-V_Parental_A_background_normalized.profile.wig.gz |
21.2 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_Parental_A_normalized.profile.wig.gz |
22.1 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_Parental_AllPeaks.bed.gz |
41.7 Kb |
(ftp)(http) |
BED |
| GSE155024_RpoD-V_Parental_B_background_normalized.profile.wig.gz |
21.0 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_Parental_B_normalized.profile.wig.gz |
22.8 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_Parental_C_background_normalized.profile.wig.gz |
21.2 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_Parental_C_normalized.profile.wig.gz |
22.2 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_delUT_A_background_normalized.profile.wig.gz |
21.5 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_delUT_A_normalized.profile.wig.gz |
22.2 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_delUT_AllPeaks.bed.gz |
36.0 Kb |
(ftp)(http) |
BED |
| GSE155024_RpoD-V_delUT_B_background_normalized.profile.wig.gz |
21.9 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_delUT_B_normalized.profile.wig.gz |
21.6 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_delUT_C_background_normalized.profile.wig.gz |
21.7 Mb |
(ftp)(http) |
WIG |
| GSE155024_RpoD-V_delUT_C_normalized.profile.wig.gz |
21.8 Mb |
(ftp)(http) |
WIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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