|
| Status |
Public on May 05, 2009 |
| Title |
Using high-density exon arrays to profile gene expression in closely related species (Exon 1.0 ST) |
| Platform organism |
Homo sapiens |
| Sample organism |
Pan troglodytes |
| Experiment type |
Expression profiling by array
|
| Summary |
Global comparisons of gene expression profiles between species provide significant insight into gene regulation, evolutionary processes, and disease mechanisms. In this work, we describe a flexible and intuitive approach for global expression profiling of closely related species, using high-density exon arrays designed for a single reference genome. The high-density probe coverage of exon arrays allows us to select the identical set of perfect-match probes for measuring expression levels of orthologous genes. This eliminates a serious confounding factor in probe affinity effects of species-specific microarray probes, and enables direct comparisons of estimated expression indexes across species.
Keywords: analysis of gene expression in tissues
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| |
|
| Overall design |
We conducted Human Exon 1.0 array profiling of RNAs from three chimpanzee cerebellums and three chimpanzee livers
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| |
|
| Contributor(s) |
Xing Y |
| Citation(s) |
19474342, 20864445 |
| |
| Submission date |
Apr 10, 2009 |
| Last update date |
Nov 05, 2019 |
| Contact name |
Yi Xing |
| E-mail(s) |
[email protected]
|
| Phone |
319-384-3099
|
| Fax |
319-384-3150
|
| Organization name |
University of Iowa
|
| Department |
Internal Medicine, Biomedical Engineering
|
| Street address |
3294 CBRB, 285 Newton Rd
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
| GSM391230 |
Chimpanzee cerebellum, biological rep 1 |
| GSM391231 |
Chimpanzee liver, biological rep 1 |
| GSM391232 |
Chimpanzee cerebellum, biological rep 2 |
| GSM391233 |
Chimpanzee liver, biological rep 2 |
| GSM391234 |
Chimpanzee cerebellum, biological rep 3 |
| GSM391235 |
Chimpanzee liver, biological rep 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE15666 |
Using high-density exon arrays to profile gene expression in closely related species |
|
| Relations |
| BioProject |
PRJNA122935 |
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