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| Status |
Public on Apr 29, 2021 |
| Title |
ChIP-seq data in A673 Ewing cell line (siCT & siSA2) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
STAG2, a member of cohesin, is one of the most recurrently mutated genes in human cancer. Here, we investigated STAG2 function in the context of Ewing sarcoma, an aggressive bone tumor driven by EWS-FLI1 oncogene chimeric transcription factor. We transfected A673 cell line with siCT or 2 differents siSTAG2 (siSA2#6 or siSA2#8) during 72h. The cells were profiled by ChIP-seq for EWS-FLI1, CTCF, cohesin members and H3K27ac marks and highlighted a global conservation of binding for these marks upon STAG2 knock-down
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| Overall design |
ChIP-seq were performed in A673 Ewing sarcoma cell line. Cells were transfected during 72h with either siCT, siSA2#6 or siSA6#8. For each siRNA, we performed ChIP-seq for CTCF, cohesin members (RAD21, SMC1A, STAG1 and STAG2), EWS-FLI1 and H3K27ac. Input of A673 WT was used as normalization controls.
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| Contributor(s) |
Surdez D, Zaidi S, Grossetête S, Raynal V, Baulande S, Hill V, Delattre O |
| Citation(s) |
33930311 |
| |
| Submission date |
Aug 21, 2020 |
| Last update date |
Jul 29, 2021 |
| Contact name |
Olivier Delattre |
| Organization name |
Institut Curie
|
| Department |
U830
|
| Lab |
Olivier Delattre
|
| Street address |
26 rue d'ulm
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| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE133228 |
STAG2 promotes CTCF-anchored loop extrusion and cis-promoter and -enhancer interactions |
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| Relations |
| BioProject |
PRJNA658585 |
| SRA |
SRP278403 |
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