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| Status |
Public on Jan 05, 2011 |
| Title |
Expression data from rapamycin treatment and/or p73 knock-down in Rh30 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
p73 is a p53 family transcription factor that plays critical roles during development and tumor suppression. We analyzed p73 activity using a combination of ChIP-on-Chip and gene expression profiling, both at baseline and after treatment with the mTOR inhibitor rapamycin.
We generated an mTOR-p73 gene signature that predicts rhabdomyosarcoma tumor subtype and patient outcome, and is enriched for p73 target genes involved in mesenchymal stem cell differentiation and tumorigenesis.
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| Overall design |
Rh30 rhabdomyosarcoma cells were infected with lentivirus (either control or expressing one of two RNAi constructs targeting p73) for 3 d, and treated with vehicle or 40 nM rapamycin for 24 h, and then total RNA was harvested. Experiments were performed in duplicate for a total of 8 samples. For p73 RNAi, a different targeting construct was used for each replicate.
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| Contributor(s) |
Rosenbluth JM, Pietenpol JA |
| Citation(s) |
21245298 |
| |
| Submission date |
Apr 16, 2009 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Jennifer Pietenpol |
| Organization name |
Vanderbilt University Medical Center
|
| Department |
Biochemistry
|
| Lab |
652 PRB
|
| Street address |
23rd Ave South at Pierce
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
|
| GSM393164 |
Vehicle control, sh p73-2, B |
| GSM393165 |
40 nM rapamycin, sh GFP, A |
| GSM393166 |
40 nM rapamycin, sh GFP, B |
| GSM393167 |
40 nM rapamycin, sh p73-1, A |
| GSM393168 |
40 nM rapamycin, sh p73-2, B |
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| This SubSeries is part of SuperSeries: |
| GSE15719 |
p73 activity and rapamycin treatment: ChIP-on-Chip and gene expression profiling studies |
|
| Relations |
| BioProject |
PRJNA123021 |
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