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| Status |
Public on Sep 08, 2021 |
| Title |
Generation of human blastocyst-like structures from pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Due to ethical concerns and restricted access to human blastocysts, we lack a comprehensive understanding of early human embryogenesis. A reliable model system that can recapitulate early stages of human embryogenesis would help solve this problem.Here we report a robust three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids also exhibited the developmental potential to undergo post-implantation morphogenesis in vitro to form structures with a cellular composition and transcriptome signature similar to human embryos that had been cultured in vitro for 8 or 10 days. In conclusion, human EPS-blastoids provide a robust new experimental platform for studying early developmental stages of the human embryo.
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| Overall design |
To analyze the transcriptome signature of EPS-blasotids on different developmental days (including day6, day8 and day10).
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| Contributor(s) |
Min Z, Fan Y, Yu Y |
| Citation(s) |
34489415 |
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| Submission date |
Oct 03, 2020 |
| Last update date |
Sep 08, 2021 |
| Contact name |
zheying min |
| E-mail(s) |
[email protected]
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| Organization name |
Peking University
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| Street address |
Yiheyuan road NO.5
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| City |
beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA667174 |
| SRA |
SRP286266 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE158971_RAW.tar |
397.6 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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