|
| Status |
Public on Jun 25, 2023 |
| Title |
Datasets for the effects of RUNX2 silencing on transcriptomic and metabolomic profiles in SJSA-1 osteosarcoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Osteosarcoma is the most common primary malignant bone tumor with a high risk of metastasis and recurrence. Metabolic reprogramming is a hallmark of osteosarcoma and other cancers and is associated with genetic and epigenetic alterations. RUNX2 is an important transcription factor for osteoblastic differentiation, and aberrant expression of the gene contributes to the development and progression of osteosarcoma. To identify the effects of RUNX2 silencing on transcriptomic and metabolomic profiles in osteosarcomas, we generated SJSA-1 osteosarcoma cells stably expressing RUNX2 shRNA and SJSA-1 cells stably expressing scramble shRNA and analyzed transcriptome and metabolome profiles in the two cell types using Illumina NovaSeq 6000 and ultrahigh-performance liquid chromatography coupled with time-of-flight mass spectrometry, respectively. The datasets can be used by researchers to identify novel targets of RUNX2 and elucidate the role and underlying mechanism of RUNX2 in osteosarcoma pathogenesis and metabolic reprogramming.
|
| |
|
| Overall design |
Comparing the mRNA profilings of SJSA-1 cells stably expressing RUNX2 shRNA or scramble shRNA.
|
| Web link |
https://linkinghub.elsevier.com/retrieve/pii/S2352340923006005
|
| |
|
| Contributor(s) |
Le M, Chen R, Xiang L, Zhou J, Ning Y |
| Citation(s) |
37663774 |
| |
| Submission date |
Oct 04, 2020 |
| Last update date |
Sep 24, 2023 |
| Contact name |
Jianlin Zhou |
| E-mail(s) |
[email protected]
|
| Phone |
+8613755191079
|
| Organization name |
Hunan Normal University
|
| Department |
College of Life Science
|
| Street address |
36 Lushan road
|
| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410081 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (12)
|
| GSM4816855 |
SJSA-1 cells overexpressing scramble shRNA rep1 |
| GSM4816856 |
SJSA-1 cells overexpressing scramble shRNA rep2 |
| GSM4816857 |
SJSA-1 cells overexpressing scramble shRNA rep3 |
| GSM4816858 |
SJSA-1 cells overexpressing scramble shRNA rep4 |
| GSM4816859 |
SJSA-1 cells overexpressing scramble shRNA rep5 |
| GSM4816860 |
SJSA-1 cells overexpressing scramble shRNA rep6 |
| GSM4816861 |
SJSA-1 cells overexpressing RUNX2 shRNA rep1 |
| GSM4816862 |
SJSA-1 cells overexpressing RUNX2 shRNA rep2 |
| GSM4816863 |
SJSA-1 cells overexpressing RUNX2 shRNA rep3 |
| GSM4816864 |
SJSA-1 cells overexpressing RUNX2 shRNA rep4 |
| GSM4816865 |
SJSA-1 cells overexpressing RUNX2 shRNA rep5 |
| GSM4816866 |
SJSA-1 cells overexpressing RUNX2 shRNA rep6 |
|
| Relations |
| BioProject |
PRJNA667241 |
| SRA |
SRP286294 |
Less...
More...