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| Status |
Public on Jan 27, 2021 |
| Title |
Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination |
| Organism |
Macaca mulatta |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Vaccination against tuberculosis by intradermal Bacillus Calmette-Guérin (BCG) injection saves many lives, supposedly by inducing adaptive immune memory in lymphocytes. Epidemiologically, BCG vaccination is also associated with reduced childhood mortality unrelated to TB, which is attributed to innate immune memory, also termed trained immunity. We recently demonstrated improved protection against tuberculosis infection in highly susceptible rhesus macaques by mucosal BCG vaccination, correlating with a unique local but no peripheral immune profile. Here, we investigated local and peripheral innate immune function after intradermal versus mucosal vaccination with M. bovis BCG or the live attenuated, M. tuberculosis-derived candidate, MTBVAC. The results demonstrate an augmented frequency of trained immunity in monocytes after respiratory mucosal administration of live attenuated mycobacterial vaccines compared to intradermal immunization, with MTBVAC being equally potent as BCG. These results provide further support to strategies for improving TB vaccination and, more broadly, modulating innate immunity via mucosal surfaces.
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| Overall design |
ChIP-seq using primate cells pre-treatment or treated with BCG
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| Contributor(s) |
Vierboom MP, Domínguez-Andrés J, van der Sande M, Martens JH, Verreck FA |
| Citation(s) |
33521699 |
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https://doi.org/10.1016/j.xcrm.2020.100185
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| Submission date |
Oct 05, 2020 |
| Last update date |
Nov 11, 2021 |
| Contact name |
Joost Martens |
| E-mail(s) |
[email protected]
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| Phone |
0243780645
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| Organization name |
Radboud University
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| Department |
RIMLS
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| Lab |
Molecular Biology
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| Street address |
Geert Grooteplein 28
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| City |
Nijmegen |
| State/province |
Nederland |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL21120 |
Illumina NextSeq 500 (Macaca mulatta) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA667474 |
| SRA |
SRP286416 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159046_RAW.tar |
293.3 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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