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| Status |
Public on Apr 14, 2021 |
| Title |
Gene Expression Arrays comparing C. albicans SN250 during planktonic and biofilm growth in different media |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal was to identify genes that change their expression in biofilm vs. planktonic growth. Cultures for the extraction of total RNA under biofilm growth conditions were performed on biofilms grown on the bottom of 6-well polystyrene plates for 48 hours at 37°C and 200 rpm as previously described (Nobile et al. 2012). The media used was Spider 1% glucose, Spider and RPMI 1% glucose. Planktonic cultures for total RNA were grown in the same media by inoculating with cells from an overnight 30°C YPD culture to an OD600 of 0.05. Cultures were then grown in flasks at 37°C with shaking at 225 rpm until they reached an OD600 of 1.0.
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| Overall design |
Expression was measured relative to pooled references, two replicates of each experiment were performed.
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| |
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| Contributor(s) |
Mancera E |
| Citation(s) |
33825680 |
| |
| Submission date |
Nov 03, 2020 |
| Last update date |
Apr 17, 2021 |
| Contact name |
Eugenio Mancera |
| E-mail(s) |
[email protected]
|
| Organization name |
Cinvestav
|
| Department |
Ingenieria Genetica
|
| Street address |
Km. 9.6 Libramiento Norte Carretera Irapuato-Leon
|
| City |
Irapuato |
| State/province |
Guanajuato |
| ZIP/Postal code |
36824 |
| Country |
Mexico |
| |
|
| Platforms (1) |
| GPL16243 |
Agilent-020166 Ca21_GE_plus_tiling_2.0 (Feature Number version) |
|
| Samples (12)
|
| GSM4879197 |
C. albicans SN250 Planktonic Growth in Spider Replicate 1 |
| GSM4879198 |
C. albicans SN250 Planktonic Growth in Spider Glucose Replicate 1 |
| GSM4879199 |
C. albicans SN250 Planktonic Growth in RPMI Replicate 1 |
| GSM4879200 |
C. albicans SN250 Planktonic Growth in Spider Replicate 2 |
| GSM4879201 |
C. albicans SN250 Planktonic Growth in Spider Glucose Replicate 2 |
| GSM4879202 |
C. albicans SN250 Planktonic Growth in RPMI Replicate 2 |
| GSM4879203 |
C. albicans SN250 Biofilm Growth in Spider Replicate 1 |
| GSM4879204 |
C. albicans SN250 Biofilm Growth in Spider Glucose Replicate 1 |
| GSM4879205 |
C. albicans SN250 Biofilm Growth in RPMI Replicate 1 |
| GSM4879206 |
C. albicans SN250 Biofilm Growth in Spider Replicate 2 |
| GSM4879207 |
C. albicans SN250 Biofilm Growth in Spider Glucose Replicate 2 |
| GSM4879208 |
C. albicans SN250 Biofilm Growth in RPMI Replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE160783 |
Evolution of biofilm formation in Candida |
|
| Relations |
| BioProject |
PRJNA674412 |
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