|
| Status |
Public on Nov 09, 2021 |
| Title |
Vaccine-elicited CD4 T cells prevent the deletion of antiviral B cells in chronic infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CD4 T cells responses induced by LM61 immunization are heterogeneous in phenotype. The majority consists of Th1 cells, but there is also a consistent population of Tfh-differentiated cells and a minor population of Treg cells. Tfh-differentiated cells, alongside Th1 cells expand upon LCMV challenge.
|
| |
|
| Overall design |
LCMV GP66-77-specific CD4 T cells were analyzed on day 9 after LM61 immunization. Additional mice were given KL25HL B cell transfer and LCMV challenge on day 9 after LM61 immunization and GP66-77-specific CD4 T cells were analyzed on day 14. To control for the impact of LM61 pre-immunization on the differentiation of GP66-77-specific CD4 T cell responses upon LCMV challenge, an additional group of mice was given wildtype LM on day 0 instead of LM61.
|
| |
|
| Contributor(s) |
Narr K, Schreiner D, Pinschewer DD |
| Citation(s) |
34772811 |
| |
| Submission date |
Nov 12, 2020 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Daniel Pinschewer |
| Organization name |
University of Basel
|
| Department |
Department of Biomedicine
|
| Lab |
Experimental Virology
|
| Street address |
Petersplatz 10
|
| City |
Basel |
| ZIP/Postal code |
4003 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA677948 |
| SRA |
SRP292345 |
Less...
More...