|
| Status |
Public on Oct 01, 2021 |
| Title |
Knockdown of ILF2/ILF3 (NF45/NF90) in the gastric cancer cell line SGC7901 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Our data showed that lncRNA ELF3-AS1 could bind on the exon1 of ELF3-201 to form a double-stranded RNA molecule. This double-stranded RNA could interact with ILF2/ILF3 complex. To explore the biofunction of the interaction between ELF3-AS1 and ILF2/ILF3 complex, loss-of-function studies regarding ILF2 and ILF3 were performed in SGC7901 cell line. RNA sequencing studies showed that knockdown of ILF3 significantly decreased ELF3-AS1, while knockdown of ILF2 significantly increased ELF3-AS1 and NF90 expression. Our data revealed that ILF2/ILF3 complex interacted with ELF3-AS1/ELF3 double-stranded RNA and regulated their transcripts stability.
|
| |
|
| Overall design |
The biofunction of the interaction between ELF3-AS1 and ILF2/ILF3 complex, loss-of-function studies regarding ILF2 and ILF3 were performed in SGC7901 cell line. The potent ILF2/ILF3 target genes were identified by deep sequencing, using Illumina HiSeq 4000.
|
| |
|
| Contributor(s) |
Qin S, Li D |
| Citation(s) |
36457025 |
| |
| Submission date |
Nov 16, 2020 |
| Last update date |
Dec 02, 2022 |
| Contact name |
Qin Shanshan |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Medicine
|
| Street address |
Renmin Road 30, Maojian District
|
| City |
Shiyan |
| ZIP/Postal code |
442000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA678681 |
| SRA |
SRP292713 |
Less...
More...