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Series GSE16173 Query DataSets for GSE16173
Status Public on Dec 01, 2009
Title Gene expression profiling of grape-bud response to two alternative dormancy-release stimuli.
Platform organisms Vitis vinifera; Vitis riparia
Sample organism Vitis vinifera
Experiment type Expression profiling by array
Summary A grape-bud-oriented genomic platform was produced for a large-scale comparative analysis of bud responses to two stimuli of grape-bud dormancy release, hydrogen cyanamide (HC) and heat shock (HS). The results suggested considerable similarity in bud response to the stimuli, both in the repertoire of responding genes and in the temporary nature of the transcriptome reprogramming. Nevertheless, the bud response to HC was slower, more condensed and stronger, as reflected by a higher number of regulated genes and a higher intensity of regulation compared to the response to HS.
 
Overall design To facilitate large-scale comparative analysis of early changes in the bud transcriptome by cDNA microarray, HC and HS were applied to canes collected from three vineyards, located in different regions, in three different years. This experimental scheme resulted in two true biological replicates for each treatment, differing in both timing and location, and loop design of technical replicates within time series.
Consistent with our previous studies, both application of 5% Dormex (HC) and incubation for 1 h in 50oC water (HS) resulted in increased bud-break rates compared to respective controls. Bud break of HS-treated and HC-treated buds started 10 to 12 days after treatment. Three weeks after treatment, HS-treated buds exhibited 100% bud break while HC-treated buds had reached 80% bud break. The control showed significantly lower levels of bud break during this period. Bud material was collected from control, HC- and HS-treated cuttings at six time points (3, 6, 12, 24, 48 and 96 h) after the treatments and used to prepare total RNA samples.
 
Contributor(s) Or E, Ophir R
Citation(s) 19653104
Submission date May 20, 2009
Last update date Mar 21, 2012
Contact name Ron Ophir
Organization name Agricultire Research Organization
Department Fruit Tree Sciences
Lab Genomics lab
Street address POB 6
City Bet-Dagan
ZIP/Postal code 50250
Country Israel
 
Platforms (1)
GPL8560 University of Arizona Vitis buds cDNA array
Samples (66)
GSM404717 Control 6 hours comparing with control 3 hours after dormancy release Series A
GSM404718 Control 12 hours comparing with control 6 hours after dormancy release Series A
GSM404719 Control 24 hours comparing with control 12 hours after dormancy release Series A
Relations
BioProject PRJNA115059

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16173_RAW.tar 58.0 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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