|
| Status |
Public on Jun 17, 2021 |
| Title |
Transcriptional profiling of indolent and aggressive mammary lesions |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed transcriptional profiling of tumor cells from indolent and aggressive lesions isolated from a mouse model of breast cancer. To initiate formation of mammary lesions, we delivered lentiviral particles carrying constitutively-activated Erbb2 tagged with HA and GFP (caErbB2) through the lactiferous glands of adult virgin female. Mammary glands bearing lesions were visualized under a fluorescence stereoscope, and regions harboring aggressive (>2mm, comprised of invasive lesions) and indolent (<2mm, enriched for in situ lesions) lesions were dissected away from each other. Lesions from multiple animals were pooled to obtain sufficient material for downstream analysis. Following tissue digestion, GFP-expressing epithelial cells were isolated by fluorescence-activated cell sorting (FACS), and then subjected to bulk RNA-seq.
|
| |
|
| Overall design |
Transcriptional profiles of tumor cells from indolent and aggressive mouse mammary lesions were generated from multiple pools of animals
|
| |
|
| Contributor(s) |
Piwnica-Worms H, Sinha V |
| Citation(s) |
34408137 |
| |
| Submission date |
Nov 23, 2020 |
| Last update date |
Sep 08, 2021 |
| Contact name |
Yuan Qi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Texas M.D. Anderson Cancer Center
|
| Department |
Bioinformatics & Computational Biology
|
| Street address |
1400 Pressler St
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030-4008 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (15)
|
|
| This SubSeries is part of SuperSeries: |
| GSE162006 |
Profiling of indolent and aggressive mammary lesions |
|
| Relations |
| BioProject |
PRJNA680306 |
| SRA |
SRP293862 |
Less...
More...