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| Status |
Public on Apr 15, 2021 |
| Title |
BCOR modulates transcriptional activity of a subset of glucocorticoid receptor target genes involved in cell growth and mobility |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The effect of BCL6 corepressor (BCOR) on glucocorticoid signaling and was analyzed in HEK293-based cell lines using ChIP-seq and RNA-seq.
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| Overall design |
Chromatin binding of BCOR was analyzed using ChIP-seq from two biological replicates of HEK293 cells constitutively expressing GR (HEK293-GR; Paakinaho et al., 2014). Fragmented de-crosslinked chromatin was used as an input control. Gene expression was analyzed from three (rep1-3) biological replicates using RNA-seq. Poly-A RNA was extracted from BCOR depleted (siBCOR) or control siRNA-transfected (siNON) HEK293-GR cells treated with vehicle (ethanol, EtOH) or dexamethasone (100nM, 6h).
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| Contributor(s) |
Niskanen EA, Manjur KA, Palvimo JJ, Malinen M, Lempiäinen JK |
| Citation(s) |
33722704 |
| |
| Submission date |
Dec 10, 2020 |
| Last update date |
Apr 16, 2021 |
| Contact name |
Einari A Niskanen |
| E-mail(s) |
[email protected]
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| Organization name |
University of Eastern Finland
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| Department |
School of Medicine
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| Lab |
Biomedicine
|
| Street address |
Yliopistonranta 8
|
| City |
Kuopio |
| ZIP/Postal code |
70210 |
| Country |
Finland |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA684509 |
| SRA |
SRP297659 |
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