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| Status |
Public on May 03, 2021 |
| Title |
Knockdown of pseudogene lncRNA UBE2CP3 in gastric cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our data showed that UBE2CP3 was aberrantly upregulated in gastric cancer (GC), but its biofunction in GC is largely unknown to date. To explore the function of lncRNA UBE2CP3 in gastric cancer, loss-of-function and RNA sequencing studies were performed in SGC7901 cell line. The results showed that depletion of UBE2CP3 significantly decreased the expression of ITGA2. Interestingly, ITGA2 was also a target gene of miR-138. Our data showed that UBE2CP3 promotes GC progression through regulating miR-138/ITGA2 axis. Additionally, lncRNA UBE2CP3 could be stabilized by IGFBP7 mRNA. IGFBP7 depletion also significantly decreased ITGA2 expression.
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| Overall design |
To reveal the possible target genes of UBE2CP3 and miR-138 in gastric cancer, we transfected UBE2CP3-targeted siRNAs and miR-138-5p mimics in the SGC7901 and AGS cell lines. The potent UBE2CP3 and miR-138 target genes were identified by deep sequencing, using Illumina HiSeq 4000.
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| Contributor(s) |
Qin S, Li D |
| Citation(s) |
34274947 |
| |
| Submission date |
Dec 24, 2020 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Qin Shanshan |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Medicine
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| Street address |
Renmin Road 30, Maojian District
|
| City |
Shiyan |
| ZIP/Postal code |
442000 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA687672 |
| SRA |
SRP299256 |
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