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| Status |
Public on May 03, 2021 |
| Title |
RIP-seq using ILF3 antibodies to reveal RNAs that binds to ILF3 protein |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
RNA pulldown assay showed that lncRNA UBE2CP3 could bind to ILF3 protein. In order to further verify the interaction between ILF3 and UBE2CP3, RNA Immunoprecipitation (RIP) assay was performed to identify the RNAs that binds to ILF3 protein. RIP-seq data verifies the interaction between ILF3 and UBE2CP3. Interestingly, UBE2CP3 could also binds to 3'UTR of IGFBP7 mRNA. Mechanismly, lncRNA UBE2CP3 and 3' UTR of IGFBP7 could forms an double-stranded RNA. Then, the RNA duplex could interact with the Double-Stranded RNA-Binding Protein ILF3.
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| Overall design |
The potent RNA was immunoprecipitated by ILF3 antibodies using RIP assay. Then, all the potent ILF3-binding target RNAs were identified by deep sequencing, using Illumina HiSeq 4000.
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| Contributor(s) |
Qin S, Li D |
| Citation(s) |
34274947 |
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| Submission date |
Dec 24, 2020 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Qin Shanshan |
| E-mail(s) |
[email protected]
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| Organization name |
Institute of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Medicine
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| Street address |
Renmin Road 30, Maojian District
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| City |
Shiyan |
| ZIP/Postal code |
442000 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA687674 |
| SRA |
SRP299258 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163815_ILF3-RIP_peaks.txt.gz |
266.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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