|
| Status |
Public on Feb 09, 2021 |
| Title |
Tumor methionine metabolites reprograms chromatin accessibilities of CD8+ T cells linking to T cell dysfunction. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
T-cell exhaustion denotes a hypofunctional state of T lymphocytes commonly found in cancer, but how tumor cells drive T-cell exhaustion remains elusive. We found oncogenic reprograming of HCC methionine recycling with elevated 5-methylthioadenosine (MTA) and S-adenosylmethionine (SAM) to be tightly linked to T-cell exhaustion. We used an assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) to assay the genome-wide chromatin accessibility of T cells during activation and SAM/MTA treatment.
|
| |
|
| Overall design |
ATAC-sequencing was performed on non-activated CD8+ T cell and activated CD8+ T cells with and without SAM or MTA treatment.
|
| |
|
| Contributor(s) |
Wang XW |
| Citation(s) |
33674593 |
| |
| Submission date |
Feb 04, 2021 |
| Last update date |
Apr 06, 2021 |
| Contact name |
Xin Wei Wang |
| E-mail(s) |
[email protected]
|
| Phone |
2407606858
|
| Organization name |
National Institutes of Health
|
| Department |
Center for Cancer Research
|
| Lab |
Laboratory of Human Carcinogenesis
|
| Street address |
37 Convent Drive, Bldg 37 Rm 3050
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA699597 |
| SRA |
SRP304779 |
Less...
More...