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| Status |
Public on Aug 04, 2021 |
| Title |
Cellular transcriptomes of wild-type and ING3 knockout HT-29 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study is to use RNA-sequencing to profile the transcriptional changes in HT-29 cells with ING3 deficiency. Specifically, we were interested in determining the IFN and ISGs in response to ING3 KO. Several ISGs including IFI44L and IFITM1 were up-regulated in ING3 KO HT-29 cells, indicating that the broad resistance to virus infection in ING3 KO cells were mediated by heightened IFN and ISG responses.
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| Overall design |
mRNA profiles of WT and ING3 KO HT-29 cells were analyzed by bulk RNA-sequencing
We used one sgRNA to knockout ING3 in HT-29 cells, then we subcloned the HT-29-ING3 KO cells. #1 and #2 mean different subclones of HT-29-ING3 KO cells.
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| Contributor(s) |
Song Y, Ding S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 04, 2021 |
| Last update date |
Aug 04, 2021 |
| Contact name |
Gaopeng Hou |
| E-mail(s) |
[email protected]
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| Phone |
3149065080
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| Organization name |
Washinton University inSt. Louis
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| Department |
molecular microbiology
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| Street address |
McDonnell Pediatric Research Building, Rm 9220
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| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA699603 |
| SRA |
SRP304782 |
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