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| Status |
Public on Feb 09, 2021 |
| Title |
Human Naïve Epiblast Cells Possess Unrestricted Lineage Potential |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Classical mouse embryology has established a paradigm of early development drivenby sequential lineage bifurcations. Accordingly, mouse embryonic stem cells derivedfrom early epiblast have lost the potency to produce extraembryonic trophectoderm.We show in contrast that human naive epiblast cells readily make trophectoderm.Inhibition of ERK signalling, instrumental in naive stem cell propagation, unexpectedlypotentiates trophectoderm formation, an effect enhanced by Nodal inhibition.Transcriptome analyses authenticate conversion into trophectoderm with subsequentproduction of syncitiotrophoblast, cytotrophoblast and trophoblast stem cells. Geneticperturbations indicate that NANOG suppresses and TFAP2C enables trophectoderminduction. Consistent with post-implantation progression, trophectoderm potential isextinguished in conventional human pluripotent stem cells, which instead makeamnion. Finally, human embryo epiblasts from late blastocysts efficiently generatetrophectoderm and differentiated trophoblast. Thus, pluripotent cells in the humanembryo retain extraembryonic lineage plasticity and regenerative potential untilimplantation. Harnessing this unanticipated regulative capacity may be beneficial forassisted reproduction technology.
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| Overall design |
The deposited data are RNAsequencing data for studying human naïve stem cells to trophectoderm differentiation. The study comprises two human naïve pluripotency cell lines, HNES1 and cR-H9. The two cell lines were treated in four different medium, N2B27 basal medium or N2B27 basal mudium supplementated with PD0325902(PD03), A8301 or PD03+A8301(PDA83). RNA samples were collected in a time course.
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| Contributor(s) |
Guo G, Stirparo GG |
| Citation(s) |
33831366 |
| |
| Submission date |
Feb 08, 2021 |
| Last update date |
Jun 08, 2021 |
| Contact name |
Giuliano Giuseppe Stirparo |
| E-mail(s) |
[email protected]
|
| Organization name |
Cerevance ltd
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| Street address |
418 Cambridge Science Park Milton Rd
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB4 0PZ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA700707 |
| SRA |
SRP305413 |
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