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| Status |
Public on Feb 18, 2021 |
| Title |
The impact of exposure to excess zinc on the transcriptome of Streptococcus mutans |
| Organism |
Streptococcus mutans UA159 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Zinc is a micronutrientient that serves as an important co-factor necessary for enzymatic function. In the host environment, access to metals can be highy restricted. As excess metals can be toxic to the bacterial cell, the host immune system can utilize zinc intoxication as a defense mechanism against bacterial infection. Here, we wanted to identify the transcriptomic response when S. mutans UA159 is exposed to an excess of zinc.
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| Overall design |
Streptococcus mutans UA159 was grown in the chemically defined medium FMC. Cultures were grown to mid-exponential phase (OD600 = 0.4). At this point, control samples were harvested for transcriptional profiling. To the remaining samples, 4mM ZnSO4 was added. The incubation of cells was continued to allow for exposure to the excess zinc for 15 or for 90 minutes, at which point these samples were harvested.
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| Contributor(s) |
Kajfasz JK, Ganguly T, Lemos J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 17, 2021 |
| Last update date |
Feb 22, 2021 |
| Contact name |
Jessica K Kajfasz |
| E-mail(s) |
[email protected]
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| Organization name |
University of Florida
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| Department |
Oral Biology
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| Lab |
Lemos-Abranches
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| Street address |
1395 Center Drive
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL29159 |
Illumina NextSeq 500 (Streptococcus mutans UA159) |
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| Samples (8)
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| GSM5090455 |
S. mutans UA159 grown to OD600 = 0.4 in FMC complete Replicate 1 |
| GSM5090456 |
S. mutans UA159 grown to OD600 = 0.4 in FMC complete Replicate 2 |
| GSM5090457 |
S. mutans UA159 grown to OD600 = 0.4 in FMC complete Replicate 3 |
| GSM5090458 |
S. mutans UA159 grown in FMC to OD600 = 0.4, then exposed to 4mM ZnSO4 for 15 minutes Replicate 1 |
| GSM5090459 |
S. mutans UA159 grown in FMC to OD600 = 0.4, then exposed to 4mM ZnSO4 for 15 minutes Replicate 2 |
| GSM5090460 |
S. mutans UA159 grown in FMC to OD600 = 0.4, then exposed to 4mM ZnSO4 for 90 minutes Replicate 1 |
| GSM5090461 |
S. mutans UA159 grown in FMC to OD600 = 0.4, then exposed to 4mM ZnSO4 for 90 minutes Replicate 2 |
| GSM5090462 |
S. mutans UA159 grown in FMC to OD600 = 0.4, then exposed to 4mM ZnSO4 for 90 minutes Replicate 3 |
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| Relations |
| BioProject |
PRJNA702540 |
| SRA |
SRP306902 |
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