|
| Status |
Public on May 04, 2021 |
| Title |
Comparison of spatial sequencing in leprosy lesions from lepromatous and reversal reaction patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Granulomas are complex cellular structures comprised predominantly of macrophages and lymphocytes that function to contain and kill invading pathogens. Here, we investigated single cell phenotypes associated with antimicrobial responses in human leprosy granulomas by applying single cell and spatial sequencing to leprosy biopsy specimens. We focused on reversal reactions (RR), a dynamic process in which some patients with disseminated lepromatous leprosy (L-lep) transition towards self-limiting tuberculoid leprosy (T-lep), mounting effective antimicrobial responses. We identified a set of genes encoding proteins involved in antimicrobial responses that are differentially expressed in RR versus L-lep lesions, and regulated by IFN- and IL-1. By integrating the spatial coordinates of the key cell types and antimicrobial gene expression in RR and T-lep lesions, we constructed a map revealing the organized architecture of granulomas depicting compositional and functional layers by which macrophages, T cells, keratinocytes and fibroblasts contribute to the antimicrobial response.
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| |
|
| Overall design |
Examine differences between Lepromatous and Reversal reaction lesions by spatial sequencing
|
| |
|
| Contributor(s) |
Ma F, Plazyo O, Gudjonsson JE, Pellegrini M, Modlin RL |
| Citation(s) |
34168371 |
| |
| Submission date |
Feb 28, 2021 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Feiyang Ma |
| Organization name |
UCLA
|
| Department |
Molecular Biology Institute
|
| Lab |
Pellegrini Lab
|
| Street address |
610, Charles Young Dr East, TLSB 3000C
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
| GSM5115019 |
spatial_transcriptomics_Lepromatous 1 |
| GSM5115020 |
spatial_transcriptomics_Lepromatous 2 |
| GSM5115021 |
spatial_transcriptomics_Reversal reaction 1 |
| GSM5115022 |
spatial_transcriptomics_Reversal reaction 2 |
| GSM5115023 |
spatial_transcriptomics_Reversal reaction 3 |
| GSM5115024 |
spatial_transcriptomics_Tuberculoid 1 |
|
| Relations |
| BioProject |
PRJNA705463 |
| SRA |
SRP308558 |
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