|
| Status |
Public on Mar 08, 2022 |
| Title |
The ACF chromatin remodeling complex is essential for Polycomb repression |
| Organism |
Neurospora crassa |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
Establishing and maintaining appropriate gene repression is critical for the health and development of multicellular organisms. Histone H3 lysine 27 (H3K27) methylation is a chromatin modification associated with repressed facultative heterochromatin, but the mechanism of repression remains unclear. We used a forward genetic approach to identify novel genes involved in transcriptional silencing of H3K27 methylated chromatin in the filamentous fungus, Neurospora crassa. We found crf4-1Iswi and crf4-2Itc1/Acf1 are required for repression of a subset of H3K27 methylated genes and that their protein products interact to form an ACF-like chromatin remodeling complex. This N. crassa ACF-like complex is promiscuous, yet can target facultative heterochromatin, where it is responsible for pulling the +1 nucleosome in the 5’ direction. H3K27 methylated genes that are up-regulated when crf4-1Iswi or crf4-2Itc1/Acf1 are deleted show a downstream shift of the +1 nucleosome suggesting that proper nucleosome positioning is important for repression of facultative heterochromatin.
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| |
|
| Overall design |
MNase-seq, H3K27 methylation ChIP-seq, RNA-seq and DamID with Neurospora crassa.
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| |
|
| Contributor(s) |
Wiles ET, Mumford C, McNaught KJ, Tanizawa H, Selker EU |
| Citation(s) |
35257662 |
| |
| Submission date |
Mar 04, 2021 |
| Last update date |
Mar 11, 2022 |
| Contact name |
Hideki Tanizawa |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Oregon
|
| Department |
Institute of Molecular Biology
|
| Street address |
1370 Franklin Blvd
|
| City |
Eugene |
| State/province |
OR |
| ZIP/Postal code |
97403 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL23150 |
Illumina HiSeq 4000 (Neurospora crassa) |
| GPL26551 |
Illumina NovaSeq 6000 (Neurospora crassa) |
|
| Samples (59)
|
|
| Relations |
| BioProject |
PRJNA706722 |
| SRA |
SRP309347 |
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