|
| Status |
Public on Apr 01, 2021 |
| Title |
Defining the transcriptional regulon of ChvI in Caulobacter crescentus |
| Organism |
Caulobacter vibrioides |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sought to define the transcriptional regulon of the ChvI response regulator in Caulobacter crescentus. This study compares gene expression between ∆chvI cells and cells overexpressing the constitutively-active chvI(D52E) mutant. Our work provides a global view of the genes directly and indirectly regulated by the ChvGI two-component system in C. crescentus.
|
| |
|
| Overall design |
6 replicates of Caulobacter cresentus ∆chvI EV and ∆chvI chvI(D52E)++ cultures were grown in PYE + 0.15% xylose before total RNA extraction using TRIzol. RNA-seq reads were mapped to the NA1000 reference genome (CP001340).
|
| |
|
| Contributor(s) |
Stein BJ, Fiebig A, Crosson S |
| Citation(s) |
34124942 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM131762 |
Molecular mechanisms controlling stress responses and cell adhesion in bacteria |
MICHIGAN STATE UNIVERSITY |
Sean Crosson |
|
| |
| Submission date |
Mar 15, 2021 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Sean Crosson |
| E-mail(s) |
[email protected]
|
| Phone |
5178845345
|
| Organization name |
Michigan State University
|
| Department |
Dept. Microbiology and Molecular Genetics
|
| Street address |
567 Wilson Rd
|
| City |
East Lansing |
| State/province |
Michigan |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24555 |
Illumina NextSeq 500 (Caulobacter vibrioides) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA714670 |
| SRA |
SRP310751 |
Less...
More...