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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 05, 2022 |
| Title |
Liver gene expression profile of wild type, liver-specific PTEN KO, SCAP KO and PTEN/SCAP double KO mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Nonalcoholic steatohepatitis (NASH), a severe form of nonalcoholic fatty liver disease, is characterized by hepatic steatosis and hepatocellular injury and progresses to cirrhosis and hepatocellular carcinoma. Sterol regulatory element-binding proteins (SREBPs) are master regulators of lipogenesis. Liver-specific PTEN knockout (KO) mice show constitutive upregulation of SREBP through PI3K-Akt pathway activation, leading to spontaneous fatty liver and subsequent HCC development. SREBP cleavage-activating protein (SCAP) plays a critical role in SREBP activation. We sought to determine the impact of SREBP inhibition on NASH and HCC development. To this end, we additionally inhibited SREBP pathway in liver-specific PTEN mice by ablating SCAP and generated liver-specific PTEN/SCAP double KO (DKO) mice. However unexpectedly, inhibition of SCAP/SREBP pathway markedly exacerbated liver injury (5weeks), fibrosis (5months), and carcinogenesis (7 months) in PTEN KO mice. To elucidate the mechanisms of liver injury in liver-specific PTEN/SCAP DKO mice, we conducted transcriptome analyses of the livers.
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| Overall design |
PTEN F/F, SCAP F/F and PTEN/SCAP F/F mice were crossed to Alb-Cre mice to generate liver-specific PTEN KO, SCAP KO and PTEN/SCAP DKO mice, respectively. Wilt type, liver-specific PTEN KO, SCAP KO and PTEN/SCAP DKO mice were fed with normal diet for 5 weeks, and total RNA samples were extracted from liver tissues. We compared relative liver gene expression levels across these mice.
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| Contributor(s) |
Kawamura S, Fujiwara N, Hoshida Y, Nakagawa H |
| Citation(s) |
35380992 |
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| Submission date |
Mar 17, 2021 |
| Last update date |
Apr 07, 2022 |
| Contact name |
Naoto Fujiwara |
| E-mail(s) |
[email protected]
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| Organization name |
Mie University
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| Street address |
2-174 Edobashi
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| City |
Tsu city |
| State/province |
Mie |
| ZIP/Postal code |
514-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| GSM5176423 |
Liver tissue of mouse with liver-specific double knockout of Pten and Scap 1 |
| GSM5176424 |
Liver tissue of mouse with liver-specific double knockout of Pten and Scap 2 |
| GSM5176425 |
Liver tissue of mouse with liver-specific double knockout of Pten and Scap 3 |
| GSM5176426 |
Liver tissue of mouse with liver-specific knockout of Pten 1 |
| GSM5176427 |
Liver tissue of mouse with liver-specific knockout of Pten 2 |
| GSM5176428 |
Liver tissue of mouse with liver-specific knockout of Pten 3 |
| GSM5176429 |
Liver tissue of mouse with liver-specific knockout of Scap 1 |
| GSM5176430 |
Liver tissue of mouse with liver-specific knockout of Scap 2 |
| GSM5176431 |
Liver tissue of mouse with liver-specific knockout of Scap 3 |
| GSM5176432 |
Liver tissue of wild-type mouse 1 |
| GSM5176433 |
Liver tissue of wild-type mouse 2 |
| GSM5176434 |
Liver tissue of wild-type mouse 3 |
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| Relations |
| BioProject |
PRJNA715216 |
| SRA |
SRP311091 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE169104_PtenScap_RLE_symbolmed_num0in50per_log2_cv0.01_unlog.gct.gz |
1.3 Mb |
(ftp)(http) |
GCT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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