|
| Status |
Public on Mar 24, 2021 |
| Title |
Remodeling of Mouse Hippocampus Genomic Fabrics in Neuropsychiatric Systemic Lupus Erythematosus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
TNF-like weak inducer of apoptosis (TWEAK) and its cognate receptor Fn14 have been shown to play an important role in neurocognitive dysfunction in murine lupus. We profiled and compared gene expression in the hippocampi of MRL/+, MRL/lpr and MRL/lpr-Fn14 knockout (Fn14ko) adult female mice to determine the transcriptomic impact of TWEAK/Fn14 on hippocampal gene expression in lupus. We found that the TWEAK/Fn14 pathway strongly affects the expression level, variability and coordination of the genomic fabrics responsible for neurotransmission and chemokine signaling. Dysregulation of the PI3K-Akt pathway in the MRL/lpr lupus strain compared with the MRL/+ control and Fn14ko mice was particularly prominent and therefore promising as a potential therapeutic target, although the complexity of the transcriptomic fabric highlights important considerations in in vivo experimental models.
|
| |
|
| Overall design |
Comparison of gene expression profiles in hippocampi of MRL/lpr, Fn14ko and MRL/MpJ adult female mice
Please note that [1] multiple yellow strategy was used to hybridize differently labelleld biological replicas with the microarray and then iteratively normalizing the expression data to the intra-array and inter-array median expression until the maximum error of estimate becomes less than 5% [2] Each raw data file contains raw data for two samples as indicated in the corresponding sample description field.
|
| |
|
| Contributor(s) |
Iacobas DA, Wen J, Iacobas S, Schwartz N, Putterman C |
| Citation(s) |
34440346 |
| |
| Submission date |
Mar 23, 2021 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Dumitru Andrei Iacobas |
| E-mail(s) |
[email protected]
|
| Phone |
936-261-9926
|
| Organization name |
Prairie View A&M University
|
| Department |
Undergraduate Medical Academy
|
| Lab |
Personalized Genomics
|
| Street address |
Ann Preston St
|
| City |
Prairie View |
| State/province |
TX |
| ZIP/Postal code |
77446 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA716684 |
Less...
More...