|
| Status |
Public on Dec 03, 2021 |
| Title |
Vitamin D treatment timing is critical for transcriptome modulation of immune challenged primary human cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, the transcriptome of peripheral blood mononuclear cells (PBMCs) of a healthy adult was investigated in response to in vitro treatment with LPS, beta-glucan and 1,25(OH)2D3.
|
| |
|
| Overall design |
RNA-seq of PBMCs of one individual treated immediately after isolation in 3 biologial repeats with 10 ng/ml lipopolysaccharide (L), 5 µg/ml beta-glucan (B), their solvent (0.1% DMSO (D)), the biologically active form of vitamin D, 10 nM 1,25(OH)2D3 (V) or its solvent (0.1% ethanol (E))
|
| Web link |
http://10.3389/fimmu.2021.754056
|
| |
|
| Contributor(s) |
Carlberg C, Hanel A |
| Citation(s) |
34956186 |
| |
| Submission date |
Apr 14, 2021 |
| Last update date |
Dec 27, 2021 |
| Contact name |
Andrea Hanel |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
University of Eastern Finland
|
| Department |
Institute of Biomedicine
|
| Lab |
Prof. Carsten Carlberg Lab
|
| Street address |
Yliopistonranta 1E, POB 1627
|
| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (54)
|
|
| Relations |
| BioProject |
PRJNA721896 |
| SRA |
SRP314805 |
Less...
More...