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| Status |
Public on Sep 30, 2021 |
| Title |
Single-cell transcriptomics reveals a conserved metaplasia program in pancreatic injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Acinar to ductal metaplasia (ADM) occurs in the pancreas in response to tissue injury and is a potential precursor for adenocarcinoma. We perfomed scRNAseq on lineage-traced cells resulting from acinar cells upon pancreas injury.
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| Overall design |
Acinar cells were lineage-traced with enhanced yellow fluorescent protein (EYFP) to follow their fate upon injury. Mice were sacrificed after either 2 or 4 weeks of caerulein treatment and pancreata were collected. Two mice for each time point. Transcripts of over 13,000 EYFP+ cells were profiled. Single-cell trajectories were generated. Data were compared to gastric metaplasia and human pancreatitis.
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| Contributor(s) |
Ma Z, Lytle N, Wahl GM, DelGiorno KE |
| Citation(s) |
34695382 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 CA197687 |
Combining single cell approaches and a developmental perspective to discover stem cell control circuits and the cellular and molecular bases of cancer heterogeneity |
THE SALK INSTITUTE FOR BIOLOGICAL STUDIES |
Geoffrey Myles Wahl |
| T32 CA009370 |
Cell Regulation, Differentiation and Cancer |
THE SALK INSTITUTE FOR BIOLOGICAL STUDIES |
Geoffrey Myles Wahl |
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| |
| Submission date |
Apr 19, 2021 |
| Last update date |
Dec 30, 2021 |
| Contact name |
Zhibo Ma |
| E-mail(s) |
[email protected]
|
| Organization name |
Salk Institute for Biological Studies
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA723113 |
| SRA |
SRP315444 |
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