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| Status |
Public on Aug 13, 2021 |
| Title |
Single-cell profiling of tumor-infiltrating TCF7+ T cells reveals a T lymphocyte subset associated with tertiary lymphoid structures and superior prognosis in oral cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We isolated OSCC cells and performed single-cell RNA sequencing(scRNA-seq). Using immunohistochemistry(IHC) to analyze the relationship between TLS and prognosis. Multiple immunohistochemistry (MIHC), Flow cytometry (FCM) and Spatial analysis were used to verify the characteristics of TCF7+ T cells and their correlation with TLS. The prognostic significance, upstream regulatory network and intercellular communication of the TCF7+ T cell subpopulation were performed by multivariate analysis, Scenic and CellPhone software package analysis.We found a strong association between TCF7+ T cell subsets, TLSs and prognosis. The results suggested that TCF7+ T cells are expressing high levels of TLS-related genes and low levels of immune checkpoint molecules. Finally, we found that TCF7+ T cells were significantly associated with favorable outcomes. We also describe the upstream drivers and intercellular molecules that these cells rely on.The results showed that TCF7+ T cells could be used as a new therapeutic target to regulate the immune response of OSCC and are expected to be a new prognostic marker.
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| |
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| Overall design |
Examination of 6 different OSCC patients
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| |
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| Contributor(s) |
Li J, Fan S, Peng Y |
| Citation(s) |
34044317 |
| |
| Submission date |
Apr 21, 2021 |
| Last update date |
Aug 14, 2021 |
| Contact name |
peng yu |
| E-mail(s) |
[email protected]
|
| Phone |
13411975386
|
| Organization name |
Sun Yat-sen Memorial Hospital of Sun Yat-sen University
|
| Street address |
Department of Oral and Maxillofacial Surgery
|
| City |
guangzhou |
| ZIP/Postal code |
515041 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA723606 |
| SRA |
SRP315775 |
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