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| Status |
Public on Jul 31, 2009 |
| Title |
Transcriptome analysis of the roxSR and anr mutant strains of Pseudomonas aeruginosa under aerobic conditions |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
To assess the role of two redox-sensitive transcriptional regulators, RoxSR and ANR, in Pseudomonas aeruginosa under aerobic conditions, microarray analysis was performed. Transcriptome profiles of roxSR mutant and anr mutant aerobically grown in LB medium were determined by Affymetrix GeneChip at both the exponential phase and early stationary phase and compared to that of the wild type strain.
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| Overall design |
Pseudomonas aeruginosa wild type (PAO1ut), roxSR mutant (ROX1), and anr mutant (PAO6261) strains were cultivated aerobically in LB in Erlenmeyer flasks, and total RNAs were extracted at both the exponential phase (OD600 = 0.3) and early stationary phase (OD600 = 1.4). The experiment was performed in duplicate independent cultures.
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| Contributor(s) |
Kawakami T, Kuroki M, Ishii M, Igarashi Y, Arai H |
| Citation(s) |
19930444 |
| |
| Submission date |
Jul 24, 2009 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Hiroyuki Arai |
| Organization name |
The University of Tokyo
|
| Department |
Department of Biotechnology
|
| Lab |
Lab. of Applied Microbiology
|
| Street address |
Yayoi 1-1-1
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA119029 |
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