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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 05, 2022 |
| Title |
Liver tumor gene expression profile of liver-specific PTEN KO, PTEN/SCAP double KO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Nonalcoholic steatohepatitis (NASH), a severe form of nonalcoholic fatty liver disease, is characterized by hepatic steatosis and hepatocellular injury and progresss cirrhosis and hepatocellular carcinoma. Sterol regulatory elment-binding proteins (SREBPs) are master regulators of lipogenesis. Liver-specific PTEN knockout (KO) mice show constitutive upregulation of SREBP through PI3K-Akt pathway activation, leading to spontaneous fatty liver and subsequent HCC development. SREBP cleavage-activating protein (SCAP) plays a critical role in SREBP activation. We sought to determine the impact of SREBP inhibition on NASH and HCC development. To this end, we additionaly inhibited SREBP pathway in liver-specific PTEN mice by ablating SCAP and generated liver-specific PTEN/SCAP double KO (DKO) mice. However unexpectedly, inhibition of SCAP/SREBP pathway markedly exacerbated liver injury (5weeks), fibrosis (5months), and carcinogenesis (7 months) in PTEN KO mice. To elucidate the mechanisms of liver tumorigenesis in liver-specific PTEN/SCAP DKO mice, we conducted transcriptome analyses of the livers.
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| Overall design |
PTEN F/F and PTEN/SCAP F/F mice were crossed to Alb-Cre mice to generate liver-specific PTEN KO, SCAP KO and PTEN/SCAP DKO mice, respectively. Liver-specific PTEN KO and PTEN/SCAP DKO mice were fed with normal diet for 12 months and 7 months, respectively. Total RNA samples were extracted from liver tumor tissues. We compared relative liver tumor gene expression levels across these mice.
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| Contributor(s) |
Matsushita Y, Kawamura S, Nakagawa H |
| Citation(s) |
35380992 |
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| Submission date |
May 10, 2021 |
| Last update date |
Apr 05, 2022 |
| Contact name |
Hayato Nakagawa |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Tokyo
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| Street address |
7-3-1 Hongo Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| GSM5288481 |
Liver tumor tissue with liver-specific knockout of Pten 1 |
| GSM5288482 |
Liver tumor tissue with liver-specific knockout of Pten 2 |
| GSM5288483 |
Liver tumor tissue with liver-specific knockout of Pten 3 |
| GSM5288484 |
Liver tumor tissue with liver-specific knockout of Pten 6 |
| GSM5288485 |
Liver tumor tissue with liver-specific double knockout of Pten and Scap 4 |
| GSM5288486 |
Liver tumor tissue with liver-specific double knockout of Pten and Scap 5 |
| GSM5288487 |
Liver tumor tissue with liver-specific double knockout of Pten and Scap 6 |
| GSM5288488 |
Liver tumor tissue with liver-specific double knockout of Pten and Scap 7 |
| GSM5288489 |
Liver tumor tissue with liver-specific double knockout of Pten and Scap, crossed with Rosa26-Lox-Stop-Lox-tdTomato |
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| Relations |
| BioProject |
PRJNA728674 |
| SRA |
SRP319188 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE174173_raw_gene_counts_matrix.txt.gz |
488.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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