|
| Status |
Public on Sep 06, 2022 |
| Title |
Genome-wide maps of chromatin state in human primary CAR-T cells [ChIPseq, ATAC-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the transcriptomic and epigentic profile of human primary CD19-28z CAR-T cells.
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| |
|
| Overall design |
CRISPR gene editing was used to delete MED12 or the "safe harbor" control gene AAVS1. Gene-edited CAR-T cells were cultured in vitro for 15 days. Unstimulated cells were collected on day 15. Stimulated cells were collected after 3 hours of stimulation with plate-bound anti-idiotype antibodies specific for the CAR.
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| |
|
| Contributor(s) |
Freitas K, Belk J, Daniel B, Sandor K |
| Citation(s) |
36356142 |
| |
| Submission date |
May 11, 2021 |
| Last update date |
Dec 09, 2022 |
| Contact name |
Julia Belk |
| Organization name |
Stanford University
|
| Street address |
450 Serra Mall
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (66)
|
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| This SubSeries is part of SuperSeries: |
| GSE174282 |
Genome-wide maps of chromatin state in human primary CAR-T cells |
|
| Relations |
| BioProject |
PRJNA729086 |
| SRA |
SRP319410 |
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