|
| Status |
Public on Sep 28, 2022 |
| Title |
Bacterial transcription factors binding to coding regions regulate the internal cryptic promoters |
| Organisms |
Pseudomonas aeruginosa; Pseudomonas syringae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To analyze the antisense transcripts, we performed LM-seq for Pesudomonas aeruginosa ∆algR and Pesudomonas syringae ∆rhpS and their corresponding WT strain. Date-minging in ChIP-seq and HT-SELEX results from public dataset, we got the TF binding sites (TFBS) across genome. Coupling analysis with LM-seq and these TFBS, our results revealed that the coding region binding TFs regulate antisense RNA transcription by regulating the activity of cryptic promoters.
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| |
|
| Overall design |
Pesudomonas aeruginosa ∆algR and Pesudomonas syringae ∆rhpS as well as their corresponding WT strain.
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| |
|
| Contributor(s) |
Hua C, Huang J, Wang T, Deng X |
| Citation(s) |
36200779 |
| |
| Submission date |
May 31, 2021 |
| Last update date |
Dec 28, 2022 |
| Contact name |
Xin DENG |
| E-mail(s) |
[email protected]
|
| Phone |
852-34425693
|
| Organization name |
City University of Hong Kong
|
| Department |
Department of Biomedical Sciences
|
| Lab |
Dr Xin DENG's Lab
|
| Street address |
1/F, Block 1, To Yuen Building, City University of Hong Kong, 31 To Yuen Street, Kowloon Tong
|
| City |
Hong Kong |
| ZIP/Postal code |
000000 |
| Country |
Hong Kong |
| |
|
| Platforms (2) |
| GPL28819 |
HiSeq X Ten (Pseudomonas aeruginosa) |
| GPL30212 |
HiSeq X Ten (Pseudomonas syringae) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA734038 |
| SRA |
SRP322028 |
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