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| Status |
Public on Jun 11, 2021 |
| Title |
Transcriptomic analysis of biofilm formation in persistent and transient Listeria monocytogenes from the retail deli environment |
| Organisms |
Listeria monocytogenes; Listeria innocua |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Persistence of Listeria monocytogenes in retail deli environments is a serious food safety issue, potentially leading to cross-contamination of ready-to-eat foods such as deli meats, salads, and cheeses. We previously discovered strong evidence of L. monocytogenes persistence in delis across multiple states. We hypothesized that this was correlated with isolates’ innate characteristics, such as biofilm-forming capacity or gene differences.We further chose four isolates for RNA-sequencing analysis and compared their global biofilm transcriptome to their global planktonic transcriptome. Analysis of biofilm vs planktonic gene expression did not show the expected differences in gene expression patterns. Overall, L. monocytogenes persistence in the deli environment is likely a matter of poor sanitation and/or facility design, rather than isolates’ biofilm-forming capacity, sanitizer tolerance, or genomic content
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| Overall design |
4 isolates were chosen for transcriptional analysis. For each isolate RNA was extracted in biological triplicate from 3-day old biofilms grown on stainless steel and from early stationary phase planktonic cultures as a control.
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| Contributor(s) |
Andrea J. E, Haley F. O, Clara A |
| Citation(s) |
33227214 |
| |
| Submission date |
Jun 10, 2021 |
| Last update date |
Sep 10, 2021 |
| Contact name |
Andrea Etter |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Vermont
|
| Department |
Nutrition and Food Science
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| Lab |
Food Microbiology Lab
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| Street address |
109 Carrigan Drive
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| City |
Burlington |
| State/province |
VT |
| ZIP/Postal code |
05405 |
| Country |
USA |
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| Platforms (2) |
| GPL21330 |
Illumina HiSeq 2500 (Listeria monocytogenes) |
| GPL30260 |
Illumina HiSeq 2500 (Listeria innocua) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA736746 |
| SRA |
SRP323652 |
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