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| Status |
Public on Aug 13, 2021 |
| Title |
Reconstructing aspects of human embryogenesis with pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have established an in vitro model that reconstructs aspects of human embryogensis using human expanded potential pluripotent stem cells (hEPSCs). We performed single-cell RNA sequencing (scRNA-seq) on hEPSCs grown in 2D (prior to aggregation), hEP-structures at day 5, hEP-structures grown in IVC for 24h, and natural human blastocysts at day 5/6.
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| Overall design |
Single cell transcriptome analysis of hEPSCs grown in 2D (2 replicates), hEP-structures at Day 5 (3 replicates), hEP-structures at Day 6 (3 replicates), and D5/D6 natural human embryos (6 embryos).
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| Contributor(s) |
Jorgensen V, Sozen B, Chen S |
| Citation(s) |
34548496 |
| |
| Submission date |
Jun 16, 2021 |
| Last update date |
Sep 24, 2021 |
| Contact name |
Victoria Jorgensen |
| E-mail(s) |
[email protected]
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| Organization name |
California Institute of Technology
|
| Department |
Biology and Bioengineering
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| Lab |
Zernicka-Goetz
|
| Street address |
1200 E California Blvd.
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (2) |
| GSM5387817 |
hEPSCs, D5 hEP-structures, D6 hEP-structures (multiplexed samples) |
| GSM5387818 |
D5/D6 Natural human embryos |
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| Relations |
| BioProject |
PRJNA738498 |
| SRA |
SRP324429 |
