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| Status |
Public on Jun 25, 2021 |
| Title |
Multifactorial role of mitochondria in echinocandin tolerance revealed by transcriptome analysis of drug-tolerant cells |
| Organism |
Nakaseomyces glabratus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study compared the transcriptional response of Candida glabrata echinocandin tolerant cells relative to no treated controls.
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| Overall design |
We cultured C. glabrata in YPD containing 0.06 µg/ml micafungin or 0.25 µg/ml caspofungin for 24 hours, harvested the cells, used fluorescence-activated cell sorting to enrich for echinocandin-tolerant cells, followed by modified single cell RNA sequencing at the Columbia University Genome Center. Untreated stationary or logarithmic phase cells were used as controls.
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| Contributor(s) |
Garcia-Rubio R, Shor E, Perlin DS |
| Citation(s) |
34372698 |
| |
| Submission date |
Jun 23, 2021 |
| Last update date |
Sep 14, 2021 |
| Contact name |
Rocio Garcia-Rubio |
| E-mail(s) |
[email protected]
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| Organization name |
Center for Discovery and Innovation, Hackensack Meridian Health
|
| Street address |
111 Ideation way
|
| City |
Nutley |
| State/province |
New Jersey |
| ZIP/Postal code |
07110 |
| Country |
USA |
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| Platforms (1) |
| GPL22777 |
Illumina NextSeq 500 ([Candida] glabrata) |
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| Samples (1) |
| GSM5397913 |
Drug-tolerant cells and no-drug control cells |
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| Relations |
| BioProject |
PRJNA740465 |
| SRA |
SRP325412 |
