 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 25, 2021 |
| Title |
RNA-seq profiles of rat cardiomyocytes overexpressing hsa-miR-106b-5p, hsa-miR-93-5p, hsa-miR-25-3p or cel-miR-67 |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Myocardial regeneration is restricted to early postnatal life, when mammalian cardiomyocytes still retain the ability to proliferate. The molecular cues that induce cell cycle arrest of neonatal cardiomyocytes towards terminally differentiated adult heart muscle cells remain obscure. We report that the miR-106b~25cluster is higher expressed in the early postnatal myocardium and decreases in expression towards adulthood, especially under conditions of overload, and orchestrates the transition of cardiomyocyte hyperplasia towards cell cycle arrest and hypertrophy by virtue of its targetome. To identify the relevant targets of individual miRNAs in the miR-106b~15 cluster and elucidate the molecular mechanisms underlying the proliferative effects of this microRNA cluster, we assessed the global transcriptomic changes by deep-sequencing total neonatal mouse cardiomyocyte RNA after exogeneous transfection with hsa-miR-106b-5p, hsa-miR-93-5p, hsa-miR-25-3p and compared the transcriptomic profiles to cardiomyocytes transfected with cel-miR-67, a control miRNA.
|
| |
|
| Overall design |
Cardiomyocyte RNA profiles of 1-day old wild type (WT) rats overexpressing individual members of the miR-106b~25 microRNA cluster were generated by deep sequencing using Illumina Hiseq 2000; Four condition experiment; one replicate per condition
|
| |
|
| Contributor(s) |
de Windt LJ, Dirkx E |
| Citation(s) |
34376683 |
| |
| Submission date |
Jun 24, 2021 |
| Last update date |
Sep 08, 2021 |
| Contact name |
Ryan John A Cubero |
| E-mail(s) |
[email protected]
|
| Organization name |
IST Austria
|
| Lab |
Lab Siegert
|
| Street address |
Am Campus 1
|
| City |
Klosterneuburg |
| State/province |
Lower Austria |
| ZIP/Postal code |
3400 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA741260 |
| SRA |
SRP325536 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178867_RAW.tar |
1.0 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE178867_RPKM_miRNA_All.txt.gz |
650.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...