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| Status |
Public on Jun 29, 2021 |
| Title |
FOXO3 knockdown is toxic for BL cell lines. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We found that FOXO3 knockdown induces apoptosis in BL cell lines BL-41, Namalwa and Jiyoye.
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| Overall design |
BL cell lines BL-41, Namalwa and Jiyoye were either transduced with FOXO3 shRNA or scrambled control in the pRSI12-U6-shUbiC-TagRFP lentiviral vector (BioCat, Heidelberg, Germany). Transduced cells were isolated on day 4 post transduction. RNA was isolated with the RNeasy mini kit (QIAGEN). RNA-sequencing and data processing was done by BGI (Hong-Kong). RNA libraries were prepared for sequencing using standard BGI protocols. SOAPnuke was used for data filtering and Bowtie2 was used to map clean reads to reference. RSEM was used to calculate gene expression levels and DEseq2 was used to detect DEGs. hg38 was used as reference genome.
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| Contributor(s) |
Gehringer F, Wirth T, Ushmorov A |
| Citation missing |
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| |
| Submission date |
Jun 28, 2021 |
| Last update date |
Jun 29, 2021 |
| Contact name |
Alexey Ushmorov |
| E-mail(s) |
[email protected]
|
| Phone |
+49 (0) 731 50033847
|
| Organization name |
Ulm University
|
| Department |
Institute of Physiological Chemistry
|
| Street address |
Albert-Einstein-Allee 11
|
| City |
Ulm |
| ZIP/Postal code |
89081 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA741930 |
| SRA |
SRP325840 |
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