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| Status |
Public on Nov 27, 2021 |
| Title |
Transcriptome-wide profile of 25-hydroxyvitamin D3 in primary immune cells from human peripheral blood |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we used PBMCs of five healthy individuals participating in the VitDHiD intervention trial (NCT03537027) and investigated in a transcriptome-wide approach the gene regulatory potential of 25(OH)D3 and D3 in reference to 1,25(OH)2D3 (GSE156124).
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| Overall design |
In the first experimental series (S1), PBMCs of five individuals (#05,#09,#12,#13,#14) were exposed for 24 h to either solvent (0.1% EtOH, GSE156124), 250 nM vitamin D3 (D3), 250 nM 25(OH)D3 (25D) or 10 nM 1,25(OH)2D3 (GSE156124), while in the second series (S2) PBMCs of individuals #05, #09 and #14 were stimulated for 24 h with either solvent, 100, 1000 or 10,000 nM 25(OH)D3. All experiments were performed in three repeats.
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| Contributor(s) |
Hanel A, Carlberg C |
| Citation(s) |
34836354 |
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| Submission date |
Jun 30, 2021 |
| Last update date |
Dec 01, 2021 |
| Contact name |
Andrea Hanel |
| E-mail(s) |
[email protected], [email protected]
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| Organization name |
University of Eastern Finland
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| Department |
Institute of Biomedicine
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| Lab |
Prof. Carsten Carlberg Lab
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| Street address |
Yliopistonranta 1E, POB 1627
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| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (66)
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| GSM5411746 |
Individual #05, 1000 nM 25D, rep1 |
| GSM5411747 |
Individual #05, 1000 nM 25D, rep2 |
| GSM5411748 |
Individual #05, 1000 nM 25D, rep3 |
| GSM5411749 |
Individual #05, 10000 nM 25D, rep1 |
| GSM5411750 |
Individual #05, 10000 nM 25D, rep2 |
| GSM5411751 |
Individual #05, 10000 nM 25D, rep3 |
| GSM5411752 |
Individual #05, 250 nM 25D, rep1 |
| GSM5411753 |
Individual #05, 250 nM 25D, rep2 |
| GSM5411754 |
Individual #05, 250 nM 25D, rep3 |
| GSM5411755 |
Individual #05, 250 nM D3, rep1 |
| GSM5411756 |
Individual #05, 250 nM D3, rep2 |
| GSM5411757 |
Individual #05, 250 nM D3, rep3 |
| GSM5411758 |
Individual #05, EtOH, rep1 |
| GSM5411759 |
Individual #05, EtOH, rep2 |
| GSM5411760 |
Individual #05, EtOH, rep3 |
| GSM5411761 |
Individual #09, 250 nM 25D, rep1 |
| GSM5411762 |
Individual #09, 250 nM 25D, rep2 |
| GSM5411763 |
Individual #09, 250 nM 25D, rep3 |
| GSM5411764 |
Individual #09, 250 nM D3, rep1 |
| GSM5411765 |
Individual #09, 250 nM D3, rep2 |
| GSM5411766 |
Individual #09, 250 nM D3, rep3 |
| GSM5411767 |
Individual #12, 100 nM 25D, rep1 |
| GSM5411768 |
Individual #12, 100 nM 25D, rep2 |
| GSM5411769 |
Individual #12, 100 nM 25D, rep3 |
| GSM5411770 |
Individual #12, 1000 nM 25D, rep1 |
| GSM5411771 |
Individual #12, 1000 nM 25D, rep2 |
| GSM5411772 |
Individual #12, 1000 nM 25D, rep3 |
| GSM5411773 |
Individual #12, 10000 nM 25D, rep1 |
| GSM5411774 |
Individual #12, 10000 nM 25D, rep2 |
| GSM5411775 |
Individual #12, 10000 nM 25D, rep3 |
| GSM5411776 |
Individual #12, 250 nM 25D, rep1 |
| GSM5411777 |
Individual #12, 250 nM 25D, rep2 |
| GSM5411778 |
Individual #12, 250 nM 25D, rep3 |
| GSM5411779 |
Individual #12, 250 nM D3, rep1 |
| GSM5411780 |
Individual #12, 250 nM D3, rep2 |
| GSM5411781 |
Individual #12, 250 nM D3, rep3 |
| GSM5411782 |
Individual #12, EtOH, rep1 |
| GSM5411783 |
Individual #12, EtOH, rep2 |
| GSM5411784 |
Individual #12, EtOH, rep3 |
| GSM5411785 |
Individual #13, 250 nM 25D, rep1 |
| GSM5411786 |
Individual #13, 250 nM 25D, rep2 |
| GSM5411787 |
Individual #13, 250 nM 25D, rep3 |
| GSM5411788 |
Individual #13, 250 nM D3, rep1 |
| GSM5411789 |
Individual #13, 250 nM D3, rep2 |
| GSM5411790 |
Individual #13, 250 nM D3, rep3 |
| GSM5411791 |
Individual #14, 100 nM 25D, rep1 |
| GSM5411792 |
Individual #14, 100 nM 25D, rep2 |
| GSM5411793 |
Individual #14, 100 nM 25D, rep3 |
| GSM5411794 |
Individual #14, 1000 nM 25D, rep1 |
| GSM5411795 |
Individual #14, 1000 nM 25D, rep2 |
| GSM5411796 |
Individual #14, 1000 nM 25D, rep3 |
| GSM5411797 |
Individual #14, 10000 nM 25D, rep1 |
| GSM5411798 |
Individual #14, 10000 nM 25D, rep2 |
| GSM5411799 |
Individual #14, 10000 nM 25D, rep3 |
| GSM5411800 |
Individual #14, 250 nM 25D, rep1 |
| GSM5411801 |
Individual #14, 250 nM 25D, rep2 |
| GSM5411802 |
Individual #14, 250 nM 25D, rep3 |
| GSM5411803 |
Individual #14, 250 nM D3, rep1 |
| GSM5411804 |
Individual #14, 250 nM D3, rep2 |
| GSM5411805 |
Individual #14, 250 nM D3, rep3 |
| GSM5411806 |
Individual #14, EtOH, rep1 |
| GSM5411807 |
Individual #14, EtOH, rep2 |
| GSM5411808 |
Individual #14, EtOH, rep3 |
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| Relations |
| BioProject |
PRJNA742759 |
| SRA |
SRP326374 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE179225_DGE_Ind05.xlsx |
29.8 Mb |
(ftp)(http) |
XLSX |
| GSE179225_DGE_Ind09.xlsx |
16.7 Mb |
(ftp)(http) |
XLSX |
| GSE179225_DGE_Ind12.xlsx |
27.5 Mb |
(ftp)(http) |
XLSX |
| GSE179225_DGE_Ind13.xlsx |
16.5 Mb |
(ftp)(http) |
XLSX |
| GSE179225_DGE_Ind14.xlsx |
29.2 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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